FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20141071
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC184550.064.60194120
GAGCACA285600.043.069206120
AGAGCAC390500.032.401424120
AAGAGCA633500.020.312523120
GAAGAGC687500.019.210367120
GGAAGAG882950.015.704009120
TCGGAAG967150.014.208692120
CGGAAGA1011750.013.470245118-119
ATCGGAA1174300.012.218757120
GATCGGA1174400.011.917827120
AGATCGG1267300.011.5588255120
TAGATCG322700.010.646582118-119
CTAGATC76550.09.665973116-117
TTAGATC133350.08.803652120
GAGATCG494600.08.126013118-119
AAGATCG495950.07.8509073120
CAGATCG493750.07.4996285118-119
GCCTAGA16656.5002314E-77.050853120
ATAGATC185250.06.654064120
ACTAGAT40700.06.006659114-115
Produced by FastQC (version 0.11.5)