FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15215261
Sequences flagged as poor quality0
Sequence length57-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG72300.09.377257120
TGGTCGC305650.09.088501120
AGATCTC594000.09.019878120
GATCTCG568900.08.911614120
TCTCGGT503150.08.788316120
TCGGTGG445100.08.694364120
CTCGGTG463600.08.645073120
ATCTCGG541400.08.555289120
TAGATCT616900.08.42707118-119
GGTGGTC369350.08.3495035120
GTGGTCG337850.08.311082120
GTAGATC784600.08.228101120
GGTCGCC303950.08.152364120
CGGTGGT430100.08.132701120
CGCCGTA261850.08.065396120
AGTGTAG833950.07.942637120
GAGTGTA899750.07.888575120
GTGTAGA814000.07.7392797120
AGAGTGT968450.07.5272217120
TGTAGAT846600.07.1648736120
Produced by FastQC (version 0.11.5)