FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15215261
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC171350.071.7367120
GAGCACA276600.042.04756120
AGAGCAC364200.034.09962120
AAGAGCA572650.021.663694120
GAAGAGC646950.019.712942120
GGAAGAG780200.016.202902118-119
CGGAAGA880700.014.670591120
TCGGAAG891050.014.462679120
GATCGGA1045900.012.839068120
ATCGGAA1034800.012.602149120
AGATCGG1152500.011.472878118-119
TAGATCG252750.010.211898118-119
CTAGATC67800.09.898864110-111
AAGATCG555050.09.318334116-117
TTAGATC107050.09.053679120
GAGATCG360100.08.5755625120
CAGATCG308750.08.529685120
CACACGT57550.08.362399120
TAAGATC232650.07.7788467116-117
CACGTCT58700.07.394748118-119
Produced by FastQC (version 0.11.5)