FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13913646
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC112950.058.90772120
GAGCACA171000.041.020348120
AGAGCAC227750.030.215298120
AAGAGCA380050.019.073063120
GAAGAGC410350.017.896135120
GGAAGAG503750.015.231521120
TCGGAAG571750.013.8297118-119
CGGAAGA600000.013.706091120
GATCGGA689500.012.009618120
ATCGGAA692850.011.576921120
AGATCGG755050.010.958612120
TAGATCG209250.010.014245120
CTAGATC57700.08.44194114-115
GAGATCG294050.08.024105118-119
TTAGATC107000.07.4495077112-113
AAGATCG318250.07.0816965120
CAGATCG294900.06.9125266120
ATAGATC121050.06.694234120
CCTAGAT23000.05.5016737110-111
TGAGATC149300.05.2328043114-115
Produced by FastQC (version 0.11.5)