FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15172508
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC173550.066.167206120
GAGCACA263850.043.7001120
AGAGCAC345750.034.066376120
AAGAGCA541200.022.940996120
GAAGAGC617100.020.586763120
GGAAGAG760700.016.850426120
CGGAAGA879450.014.621504118-119
TCGGAAG871700.014.467477116-117
GATCGGA1033000.012.674859120
ATCGGAA1028400.012.640242120
AGATCGG1133800.011.571669120
TAGATCG251800.011.481145120
CTAGATC62850.010.566518116-117
TTAGATC104200.09.462661120
AAGATCG482500.09.3989935114-115
GAGATCG342550.09.085684120
CACGTCT59350.09.041343120
CAGATCG366600.08.608912118-119
TAAGATC210050.08.016467114-115
CCTAGAT19350.07.801044112-113
Produced by FastQC (version 0.11.5)