FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11993644
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC135150.073.72899120
GAGCACA211750.047.815693120
AGAGCAC294500.035.72120
AAGAGCA459900.024.40038120
GAAGAGC514150.021.194958118-119
GGAAGAG627900.017.754692118-119
CGGAAGA743100.016.244186120
TCGGAAG754900.015.476456120
ATCGGAA884250.013.537735118-119
GATCGGA906950.012.874457118-119
AGATCGG994000.011.985697116-117
TAGATCG207650.011.594141120
CTAGATC54500.09.571195120
GAGATCG329650.09.392818120
AAGATCG447950.09.338166116-117
TTAGATC94650.08.973278120
CAGATCG306350.08.491635114-115
TAAGATC186900.08.015059120
CCTAGAT18650.07.888513108-109
CACGTCT36000.07.2448626120
Produced by FastQC (version 0.11.5)