FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12671055
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC192500.0100.78841120
GAGCACA304600.063.93935120
AGAGCAC413400.047.71701120
AAGAGCA599550.033.013118-119
GAAGAGC664900.028.925943116-117
GGAAGAG802550.024.391598116-117
CACACGT55950.023.692343120
CACGTCT60600.021.7214120
ACACGTC60950.021.444576120
CGGAAGA922500.020.83934114-115
TCGGAAG938600.020.6496114-115
ACGTCTG73300.020.36076120
ATCGGAA1101300.017.75055112-113
CGTCTGA81000.017.738575120
GATCGGA1131950.017.441713112-113
AGATCGG1260700.015.678942110-111
CTAGATC56550.014.901882110-111
GCACACG80250.014.55451120
TAGATCG269700.014.438344110-111
GAGATCG481050.012.66674112-113
Produced by FastQC (version 0.11.5)