FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14988020
Sequences flagged as poor quality0
Sequence length52-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT395850.08.229893120
AGAGTGT601750.07.6472316120
GTGGTCG253350.07.459584120
GTAGATC521350.07.422587120
GAGTGTA552850.07.303529120
AGTGTAG517100.07.2515435120
GTGTAGA517750.07.1845007118-119
AGATCTC402800.07.1644125120
TGGTCGC234150.07.1232076120
GATCTCG412600.07.081491120
ATCTCGG371950.07.048908120
TCTCGGT343200.06.94014120
GGTGGTC272250.06.897654120
TGTAGAT572100.06.8585258120
TCGGTGG320300.06.705807120
CGGTGGT320350.06.704761120
CTCGGTG353000.06.628494120
AAGAGTG659250.06.6207595118-119
GGTCGCC249850.06.5555367120
GGGAAAG870900.06.489445120
Produced by FastQC (version 0.11.5)