FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14988020
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC137450.055.59609120
GAGCACA207600.037.64202120
AGAGCAC264550.029.901663120
AAGAGCA433650.019.156666120
GAAGAGC457450.017.866186120
GGAAGAG562850.015.543917120
TCGGAAG632400.013.81439118-119
CGGAAGA664850.013.23621120
ATCGGAA772700.011.570979120
GATCGGA760700.011.4465885116-117
AGATCGG829950.010.718833120
TAGATCG219800.010.4534025114-115
CTAGATC56400.09.985995112-113
TTAGATC92750.08.073519116-117
AAGATCG348500.07.7131386120
CCTAGAT21400.07.47677112-113
GAGATCG337800.07.417594116-117
CAGATCG327550.06.5555005114-115
ATAGATC121750.06.2555995114-115
GCTAGAT26100.05.6399894114-115
Produced by FastQC (version 0.11.5)