FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15470561
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG427750.09.469033120
AGATCTC587150.09.034313120
GCGTCGG105000.08.91512120
CTCGGTG466500.08.849703120
TAGATCT603400.08.552342120
TCTCGGT480100.08.524022118-119
TGGTCGC294750.08.509703120
GTGGTCG322250.08.472477120
GTAGATC772600.08.341449120
AGTGTAG815700.08.290588120
ATCTCGG526250.08.27821120
GGTCGCC302550.08.270481120
GGTGGTC369800.08.161932120
GTGTAGA789750.08.160309120
GAGTGTA875350.08.136936120
CGGTGGT420650.08.088241120
GATCTCG584800.08.013746120
AGAGTGT946050.07.8142724120
AAGAGTG1027900.07.384678120
CGTATCA210350.07.21722120
Produced by FastQC (version 0.11.5)