FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12680956
Sequences flagged as poor quality0
Sequence length50-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC141550.067.28097120
GAGCACA222250.045.010212120
AGAGCAC294550.034.97172120
AAGAGCA438900.023.32323118-119
GAAGAGC502600.020.024298118-119
GGAAGAG606000.017.946283120
TCGGAAG722850.014.680557120
CGGAAGA718600.014.649603118-119
ATCGGAA853300.012.879823120
GATCGGA859750.012.830366120
AGATCGG956500.011.504148118-119
CACGTCT48000.010.842779120
CTAGATC55650.010.748754116-117
TAGATCG225500.010.58067118-119
TTAGATC80050.09.287768116-117
GAGATCG423400.08.620531120
TCTAGAT24150.08.536045114-115
CACACGT49050.08.336839118-119
AAGATCG295150.08.141077118-119
GATCTAG6500.0056194247.94737436
Produced by FastQC (version 0.11.5)