FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15625783
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC127200.043.64818120
GAGCACA173750.033.669895120
AGAGCAC217800.027.08828120
AAGAGCA359450.018.020279120
GAAGAGC397300.016.39731120
GGAAGAG495550.013.8606415120
TCGGAAG541450.013.63764120
GATCGGA634300.012.405025120
CGGAAGA595800.012.01834120
ATCGGAA665400.011.451899120
AGATCGG717250.010.827226118-119
TAGATCG170600.010.665691116-117
CTAGATC41900.010.152594116-117
TTAGATC70600.08.796212116-117
AAGATCG324400.07.5810623120
GAGATCG333150.07.3819494120
GCTAGAT19500.07.1655784114-115
CCTAGAT18150.06.3298807116-117
CAGATCG293500.06.104383118-119
CACACGT41300.06.0148616120
Produced by FastQC (version 0.11.5)