FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16051040
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC289550.0100.514366120
GAGCACA461550.064.7671120
AGAGCAC606000.048.647297118-119
AAGAGCA852100.033.6133118-119
GAAGAGC976950.029.777302116-117
GGAAGAG1149600.024.827059116-117
CGGAAGA1362400.021.305637114-115
TCGGAAG1420450.020.102736114-115
CACACGT109100.019.937883120
CACGTCT105800.019.779015120
ACACGTC111250.018.315062120
ATCGGAA1642700.017.819689112-113
ACGTCTG123300.017.195086120
GATCGGA1717850.016.94414112-113
AGATCGG1853350.015.994353110-111
CGTCTGA143750.014.876594120
TAGATCG427650.014.35387110-111
GCACACG127350.014.353465118-119
GAGATCG737600.013.71916110-111
CTAGATC86900.013.073282108-109
Produced by FastQC (version 0.11.5)