FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12661604
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC95050.038.378918120
GAGCACA126650.030.594244120
AGAGCAC166200.025.490297120
GAAGAGC300800.016.550306120
AAGAGCA298100.014.643533120
GGAAGAG385600.013.244507120
TCGGAAG415800.012.857599120
GATCGGA494900.011.570637120
AGATCGG529400.011.3029995120
CGGAAGA464450.011.114782120
TAGATCG140850.010.795133118-119
CTAGATC32600.010.625856118-119
ATCGGAA532400.010.617461120
TTAGATC66350.09.517528120
AAGATCG259400.07.941401120
TCTAGAT20100.07.6255608120
CAGATCG211350.07.3681645120
GAGATCG216850.07.2943754120
CCTAGAT15100.06.9025354114-115
GCTAGAT18000.06.8121676120
Produced by FastQC (version 0.11.5)