FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13879679
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC148500.068.44872120
GAGCACA238600.046.010414120
AGAGCAC316000.033.552677120
AAGAGCA476850.022.81797120
GAAGAGC543600.019.932127118-119
GGAAGAG657150.016.657166116-117
TCGGAAG739950.014.995982120
CGGAAGA758700.014.680366120
ATCGGAA863200.013.080339120
GATCGGA876000.012.74173118-119
AGATCGG970200.011.422212118-119
TAGATCG173600.010.749286116-117
CTAGATC48650.010.003676120
AAGATCG437900.08.967156114-115
TTAGATC73100.08.794407112-113
GAGATCG374350.08.345202116-117
TAAGATC163900.07.932445120
CAGATCG345000.07.738496120
CACGTCT47850.07.5555453120
ACACGTC49950.06.9595146120
Produced by FastQC (version 0.11.5)