FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12517091
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA1827150.015.295924120
GTATCAT789500.09.7656555120
ATCATTA685000.09.564946120
CATTAAA579800.09.5619120
TCATTAA606450.09.28022120
CGTATCA811350.09.014588118-119
CCGTATC899800.08.9953165120
TATCATT798250.08.839319120
CGCCGTA1022100.08.770163120
GCCGTAT960350.08.6468315120
TCGCCGT1128850.08.583941120
GTCGCCG1206200.08.466242120
GGTCGCC1254200.08.400558120
TGGTCGC1333450.08.2882595120
GTGGTCG1434250.07.9232903120
CGGTGGT1657900.07.712151120
ATTAAAA661350.07.711485120
GGTGGTC1531450.07.6829004120
TTAAAAA643150.07.5005693120
TCGGTGG1763100.07.3574815120
Produced by FastQC (version 0.11.5)