FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12517091
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC171300.0116.69472120
GAGCACA293250.066.723755120
AGAGCAC402300.049.644985120
AAGAGCA603800.034.084686120
GAAGAGC705300.028.888674118-119
GGAAGAG860800.023.808176118-119
CGGAAGA1006950.020.391916116-117
TCGGAAG1042050.019.628979116-117
ATCGGAA1191600.017.257801114-115
GATCGGA1219800.016.768576114-115
AGATCGG1317100.015.564288112-113
TAGATCG285100.014.364312114-115
CTAGATC71050.013.144037108-109
GAGATCG448200.012.851808112-113
TTAGATC90200.012.646869110-111
AAGATCG503000.012.519771112-113
CAGATCG392650.011.710749110-111
TAAGATC166750.011.223341114-115
CACACGT41150.010.923603120
ATAGATC137950.010.759645110-111
Produced by FastQC (version 0.11.5)