FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11632172
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC115700.056.503647120
GAGCACA185000.036.839966120
AGAGCAC239800.030.897146120
AAGAGCA377950.019.285946120
GAAGAGC392650.018.467102118-119
GGAAGAG480450.016.354664120
CGGAAGA571100.014.367012120
TCGGAAG557950.014.2525625118-119
ATCGGAA687800.012.4528120
GATCGGA685850.012.423738120
AGATCGG758300.011.419993120
TAGATCG175100.010.334773118-119
CTAGATC48750.09.846876116-117
CACACGT33700.09.18408120
AAGATCG335550.09.110823120
ACACGTC38150.08.278372120
GAGATCG281750.08.1154785120
TTAGATC74150.07.8369336120
CAGATCG264550.07.317879118-119
TCTAGAT28950.06.7635174116-117
Produced by FastQC (version 0.11.5)