FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13730384
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC150250.062.070698120
GAGCACA230600.041.129784120
AGAGCAC302400.032.84836120
AAGAGCA473500.021.954298120
GAAGAGC529300.019.41537120
GGAAGAG650800.016.064476120
TCGGAAG741900.014.109668118-119
CGGAAGA781400.013.514672120
GATCGGA899150.012.449522120
ATCGGAA894350.012.199002120
AGATCGG993300.011.34923120
TAGATCG222500.010.649358120
CTAGATC56300.09.964822120
CACGTCT48050.09.477956120
CACACGT43500.09.103764120
TTAGATC90900.08.931003120
AAGATCG430850.08.900389120
ACACGTC47700.08.440543118-119
GAGATCG335150.08.428757120
CAGATCG359900.08.19756120
Produced by FastQC (version 0.11.5)