FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14239087
Sequences flagged as poor quality0
Sequence length55-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTGT233700.09.729277120
TAGATCT141650.09.444704120
GATCTAG8951.2527235E-69.3845663
GTAGGGA405300.08.984877120
AGTGTAG196400.08.675148120
TCGTGTA555300.08.5241165120
TAGGGAA393800.08.455072120
TGTAGGG437000.08.415484120
CGTGTAG517400.08.174529120
GAGTGTA209400.08.165227120
GTGTAGG483600.08.113188120
TCTAGCC12652.3691427E-78.0624615
AGATCTC150600.07.9671884120
AAGAGTG275050.07.8521924120
CGTCGTG663050.07.6727247120
GTCGTGT588100.07.645757118-119
GTAGATC220050.07.579202120
CTAGCCG10408.298001E-57.4991426
AGCGTCG744700.07.3792834120
GTGTAGA204900.07.290502120
Produced by FastQC (version 0.11.5)