FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14239087
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC122200.045.591984120
GAGCACA174950.033.512066120
AGAGCAC216700.029.403221120
AAGAGCA356050.018.575033120
GAAGAGC375050.017.253551118-119
GGAAGAG476950.014.673023120
TCGGAAG552350.013.883132120
CGGAAGA592850.012.463794120
GATCGGA657000.012.22891120
ATCGGAA681300.012.120569120
TAGATCG184800.011.750313120
AGATCGG738250.011.23599120
CTAGATC47250.010.635712118-119
GATCTAG8607.5191565E-810.3580953
CCCTAGA9109.06457E-810.226645120
CTAGCCG9503.2074786E-79.3768016
TCTAGCC11708.198185E-99.136375
CACGTCT50000.08.685831120
TTAGATC77150.08.685027120
AAGATCG289700.08.437836120
Produced by FastQC (version 0.11.5)