FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13127394
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACCC21950.014.35332510-11
CCCTCCC40700.012.0876572
TAGGGCT29700.010.50431944-45
CGCCCTA20350.010.46610360-61
GCCCCCC11950.010.29554318-19
ATCCTAC64400.09.9698459
GCCCTCC49300.09.8574381
CCCCCCG11700.09.74610418-19
ATAGGGC34750.09.49574244-45
CCTCCCT50850.09.0849483
TGGTCGC328000.09.017665120
AACCCTA33350.08.81507968-69
CGCCGTA266750.08.546734120
GTGGTCG357150.08.466441120
CCCTCTC51950.08.4305966
GCCCTAC27800.08.4166860-61
CCCGCTA28650.08.16760826-27
CTACCCA40100.08.0818712-13
TATCCTA87200.07.9819478
GGTCGCC313250.07.967522120
Produced by FastQC (version 0.11.5)