FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13127394
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC102200.063.985126120
GAGCACA153850.039.02239120
AGAGCAC199000.031.450508118-119
AAGAGCA341300.018.108225118-119
GAAGAGC356850.017.831833116-117
CCTACCC23800.017.18926810-11
GGAAGAG455850.013.80067120
TAGGGCT31950.013.53174644-45
ATCCTAC64800.013.4326459
TCGGAAG482500.013.160661118-119
CGGAAGA518200.012.493154120
CCCTCCC41200.012.3962492
AACCCTA34750.012.11384768-69
ATAGGGC36800.011.66950144-45
CGCCCTA22100.011.35863960-61
TATCCTA80750.011.0668358
GATCGGA577000.011.062164116-117
ATCGGAA590750.010.843586112-113
ACCCTAT42100.010.660624570-71
GCCCTCC50650.010.5487131
Produced by FastQC (version 0.11.5)