FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13123824
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT248600.09.872549120
GAGTGTA362000.09.084042120
AGAGTGT402850.08.60977120
AGATCTC245450.08.532367120
GTAGATC344800.08.371146120
AGTGTAG336700.08.269552120
AAGAGTG459700.08.184651120
TCTCGGT211900.08.070882120
TAGGGAA644850.08.012197120
GTAGGGA675800.08.000438120
GTGTAGA333900.07.9614916120
ATCTCGG234950.07.713272120
GTGGTCG140250.07.6587396120
CGTGTAG821200.07.5046186120
GATCTCG252150.07.4727044120
GCGTCGG66450.07.405016120
TGTAGGG717050.07.3644514120
GGGAAAG646050.07.319261120
GGTGGTC157950.07.256394120
TCGTGTA875650.07.250405120
Produced by FastQC (version 0.11.5)