FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15500557
Sequences flagged as poor quality0
Sequence length53-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA1934550.014.730918120
GCGTCGG65900.012.108278120
GTATCAT884700.010.08396120
GCCGTAT1086100.09.876746120
CGCCGTA1134350.09.800417120
TCATTAA685250.09.700785120
CGTATCA934500.09.591521120
CATTAAA655550.09.517956120
CCGTATC1036000.09.450974120
TCGCCGT1240200.09.418688120
ATCATTA773300.09.302237120
GTCGCCG1325500.09.152034120
GGTCGCC1422200.09.006445120
TCTCGGG33250.08.841392120
TGGTCGC1532800.08.74016120
TATCATT901850.08.641537120
GTGGTCG1649450.08.602173120
GGTGGTC1772500.08.092999120
TTAAAAA694550.07.86057120
TCGGTGG2059450.07.84515120
Produced by FastQC (version 0.11.5)