FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14433415
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC188500.081.972336120
GAGCACA305650.050.018898120
AGAGCAC422100.037.817482120
AAGAGCA652350.025.148464120
GAAGAGC721000.022.499422118-119
GGAAGAG879900.018.494352118-119
CGGAAGA1046250.016.513893120
TCGGAAG1087250.015.349536118-119
ATCGGAA1266750.013.580561118-119
GATCGGA1297100.013.23093120
AGATCGG1439200.012.165995120
CACACGT51600.011.222894120
ACACGTC62950.011.039263120
TAGATCG337000.010.957337120
AAGATCG440500.010.7646265120
ACGTCTG70200.010.578523120
GAGATCG560950.010.451387118-119
CTAGATC85700.09.937992112-113
CACGTCT58300.09.87496118-119
CAGATCG418350.09.502728118-119
Produced by FastQC (version 0.11.5)