FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11641838
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC142200.073.87184120
GAGCACA225200.048.432484120
AGAGCAC308350.036.5197120
AAGAGCA467750.023.96611118-119
GAAGAGC522300.021.104885118-119
GGAAGAG645350.017.42771120
TCGGAAG749550.015.006676118-119
CGGAAGA780600.014.514774120
CACACGT41500.013.040647120
ATCGGAA884550.012.863942120
GATCGGA896300.012.719113116-117
AGATCGG977250.011.622997116-117
TAGATCG254100.011.191579110-111
ACACGTC47550.011.089595120
CTAGATC59800.010.426728114-115
CACGTCT46300.010.340004120
TTAGATC76450.09.560523114-115
GAGATCG332150.09.358288120
AAGATCG320650.09.182573114-115
CAGATCG336600.09.01502114-115
Produced by FastQC (version 0.11.5)