FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13723839
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC153300.073.477455120
GAGCACA246700.047.325268120
AGAGCAC330750.034.568302118-119
AAGAGCA500500.023.234303118-119
GAAGAGC558000.021.945002120
GGAAGAG682900.018.669172120
CGGAAGA829300.015.629245120
TCGGAAG839400.015.285174118-119
ATCGGAA989150.013.163829120
GATCGGA1013600.012.7171135116-117
AGATCGG1134050.011.657243120
TAGATCG291900.011.447202120
CACACGT39500.011.245547120
CTAGATC70000.010.68725118-119
CACGTCT47950.09.955126120
TTAGATC111750.09.093822118-119
GAGATCG435750.08.778924120
AAGATCG384800.08.37879114-115
CAGATCG399900.08.219651118-119
CCTAGAT27600.07.915852112-113
Produced by FastQC (version 0.11.5)