FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15138425
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC170150.078.67922120
GAGCACA275050.049.892597120
AGAGCAC373550.036.395954118-119
AAGAGCA587700.023.670145120
GAAGAGC643400.021.39142120
GGAAGAG824350.017.485868120
TCGGAAG963600.014.742995120
CGGAAGA965900.014.63143120
GATCGGA1156800.012.762443120
ATCGGAA1146500.012.560881120
AGATCGG1256800.011.552257118-119
TAGATCG257200.011.276638120
CTAGATC68500.010.388803108-109
AAGATCG617550.09.425922118-119
TTAGATC113550.09.164556120
GAGATCG400650.08.57151116-117
TAAGATC275950.08.320752120
CCTAGAT23200.08.2472105108-109
CAGATCG369450.07.9142423118-119
GCTAGAT26850.07.751473120
Produced by FastQC (version 0.11.5)