FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15079559
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC178700.079.58093120
GAGCACA291750.049.529995120
AGAGCAC403500.037.71768120
AAGAGCA621500.024.31539118-119
GAAGAGC691650.022.082018120
GGAAGAG840800.018.822502120
CGGAAGA1008400.015.674068120
TCGGAAG1029250.015.344279118-119
ATCGGAA1190700.013.274319120
GATCGGA1231200.012.804803120
AGATCGG1365600.011.562484118-119
TAGATCG332850.010.959877120
CTAGATC80100.010.270328120
AAGATCG559200.09.598489120
GAGATCG460800.09.5555935120
TTAGATC147300.08.76691118-119
CAGATCG400550.08.426124118-119
GGCTAGA17059.513315E-108.305235120
TAAGATC224900.08.185659118-119
ACACGTC49200.07.812081120
Produced by FastQC (version 0.11.5)