FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11393816
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC158300.083.517235120
GAGCACA259350.053.712055120
AGAGCAC347550.038.987736118-119
AAGAGCA509600.026.923399118-119
GAAGAGC604850.022.786509120
GGAAGAG725100.018.917377116-117
CGGAAGA849100.016.15848118-119
TCGGAAG857750.015.897187114-115
ATCGGAA994050.013.802813116-117
GATCGGA1015800.013.471009112-113
AGATCGG1121000.012.206613110-111
CACGTCT50350.011.88864120
CTAGATC60600.011.524875110-111
TAGATCG227250.011.431576114-115
CACACGT44000.011.420967120
ACACGTC52550.010.265895120
AAGATCG408350.09.845627110-111
TTAGATC80900.09.820748112-113
TCTAGAT24450.09.672033120
ACGTCTG69050.09.632199120
Produced by FastQC (version 0.11.5)