FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14804865
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC162800.070.006096120
GAGCACA250150.046.229855120
AGAGCAC334350.034.667816120
AAGAGCA517300.023.067257120
GAAGAGC575850.020.46605120
GGAAGAG699750.017.492989120
CGGAAGA816100.015.040068120
TCGGAAG812000.014.98929118-119
ATCGGAA966900.012.936777120
GATCGGA995700.012.576038120
AGATCGG1098900.011.422972118-119
TAGATCG242950.010.589849116-117
CTAGATC65950.010.356573120
CACACGT33650.09.153844120
AAGATCG464500.08.765673116-117
GAGATCG419250.08.139682116-117
TTAGATC106950.07.883156116-117
CAGATCG379950.07.8459134114-115
TAAGATC185300.07.2040687116-117
CACGTCT44250.06.6584044120
Produced by FastQC (version 0.11.5)