FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18545936
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC160050.047.71572120
GAGCACA232750.034.468483120
AGAGCAC295750.027.72461120
AAGAGCA505500.016.468102118-119
GAAGAGC502500.016.43075120
GGAAGAG628300.013.805027120
TCGGAAG681050.012.8763118-119
CGGAAGA723400.012.497058120
GATCGGA822500.011.829134120
ATCGGAA859650.011.222332120
AGATCGG896450.010.825116120
TAGATCG211400.010.221412118-119
CTAGATC51950.08.561751116-117
TTAGATC95050.08.247452120
CACGTCT57100.08.082337120
CACACGT52550.07.819714120
CAGATCG376700.07.5353065120
GAGATCG369050.07.451681120
AAGATCG370200.07.302469118-119
ACACGTC57700.06.9026356120
Produced by FastQC (version 0.11.5)