FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14518010
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC161350.087.06109120
GAGCACA260850.052.31661120
AGAGCAC353850.039.007076118-119
AAGAGCA536000.026.071796120
GAAGAGC609800.022.76126120
GGAAGAG725150.019.094393118-119
CGGAAGA845000.016.49821114-115
TCGGAAG858750.016.047655116-117
ATCGGAA1004350.013.851791112-113
GATCGGA1024700.013.512661114-115
AGATCGG1133800.012.279963110-111
TAGATCG244950.011.492496118-119
CTAGATC68200.010.186138108-109
AAGATCG417200.09.546176112-113
GAGATCG410200.09.079542112-113
CAGATCG438500.09.040899114-115
CACACGT35300.08.664339120
CACGTCT40950.08.535877120
TAAGATC179400.08.515036112-113
TTAGATC103750.08.378448116-117
Produced by FastQC (version 0.11.5)