FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14085157
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC116950.057.856968120
GAGCACA180350.038.407906120
AGAGCAC233650.029.460318118-119
AAGAGCA375200.019.59106120
GAAGAGC408800.018.357727120
GGAAGAG495150.015.022334118-119
TCGGAAG560000.013.538707120
CGGAAGA575400.013.399495120
GATCGGA659550.012.118166120
ATCGGAA666200.011.608232118-119
AGATCGG730500.011.081796120
CTAGATC47700.010.766807120
TAGATCG185750.010.195498120
GAGATCG280100.07.884254120
AAGATCG300800.07.741627118-119
TTAGATC87200.07.3938236114-115
CAGATCG300250.06.8847494120
TAAGATC128050.06.116395120
ATAGATC110750.06.024112118-119
AGAGATC110000.05.8902283118-119
Produced by FastQC (version 0.11.5)