FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12908225
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC121950.057.885063120
GAGCACA179850.039.99576120
AGAGCAC239850.032.52086120
AAGAGCA376050.020.190325118-119
GAAGAGC420950.018.135397118-119
GGAAGAG509300.016.469374120
TCGGAAG598900.014.581434120
CGGAAGA624400.013.863166120
GATCGGA714250.012.208673120
ATCGGAA720300.012.128402118-119
TAGATCG177400.011.163338120
AGATCGG792100.011.162002120
CTAGATC44000.010.163307118-119
TTAGATC75800.08.7228985118-119
AAGATCG368500.08.54664120
GAGATCG297350.07.884814116-117
CACACGT35900.07.473789120
CCTAGAT17150.07.263799116-117
CAGATCG291650.06.9327426116-117
GCTAGAT23650.06.7529707120
Produced by FastQC (version 0.11.5)