FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15636758
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC226200.080.16397120
GAGCACA345650.049.556065120
AGAGCAC457150.038.659996118-119
AAGAGCA685850.025.70139120
GAAGAGC758250.023.114946116-117
GGAAGAG926800.018.9788120
CGGAAGA1086150.016.409866120
TCGGAAG1107750.016.30115120
ACACGTC75400.014.0170145120
CACGTCT70100.013.999875120
ATCGGAA1316950.013.88863118-119
CACACGT69250.013.844676120
GATCGGA1328750.013.5020895116-117
AGATCGG1455000.012.607286116-117
GCACACG97800.012.041611120
TAGATCG343350.011.860126116-117
ACGTCTG92800.010.900732120
CTAGATC73800.010.607772110-111
GAGATCG529400.010.0455885112-113
CCTAGAT23350.09.83194108-109
Produced by FastQC (version 0.11.5)