FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19991658
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC386750.055.930042120
GAGCACA541800.039.69037120
AGAGCAC543450.037.160698120
AAGAGCA807500.024.7946118-119
GAAGAGC899450.021.84917118-119
CACGTCT161900.019.695375120
ACGTCTG169500.018.355467120
GGAAGAG1134250.017.575083120
ACACGTC179050.017.455069120
CGTCTGA173700.016.858013120
CACACGT186750.016.77306120
TCTGAAC172450.016.163853120
CGGAAGA1360300.015.084008120
TCGGAAG1351800.014.949031118-119
GTCTGAA204250.014.095294120
GCACACG222400.013.50934118-119
ATCGGAA1608200.012.961622118-119
GATCGGA1889850.011.99792116-117
AGATCGG2044450.011.147431116-117
TAGATCG494450.010.406576120
Produced by FastQC (version 0.11.5)