FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12794586
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC184700.094.09661120
GAGCACA305500.057.176895120
AGAGCAC416750.042.067104120
AAGAGCA614250.028.684353118-119
GAAGAGC698750.025.295595120
GGAAGAG828850.021.19933116-117
CGGAAGA954150.018.423838114-115
TCGGAAG984300.017.883852114-115
CACACGT48650.016.096254120
ACACGTC53350.015.876438120
ATCGGAA1142850.015.483192114-115
GATCGGA1166000.015.237552112-113
CACGTCT51750.014.359993120
AGATCGG1291750.013.744919112-113
TAGATCG288150.013.144341110-111
CTAGATC76700.012.553672110-111
TTAGATC105250.011.3880205110-111
AAGATCG452500.011.257403112-113
CAGATCG425450.011.174935112-113
GAGATCG425450.010.977795110-111
Produced by FastQC (version 0.11.5)