FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12405895
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC147050.073.75002120
GAGCACA219950.046.899696120
AGAGCAC302900.035.04192120
AAGAGCA483650.023.279068120
GAAGAGC511600.021.470026118-119
GGAAGAG634900.018.52438120
CGGAAGA719150.015.684184118-119
TCGGAAG731600.015.588892118-119
ATCGGAA873650.013.128033120
GATCGGA891350.012.863842116-117
AGATCGG977450.011.67836118-119
TAGATCG229150.011.047347114-115
CTAGATC58100.09.695103118-119
CACACGT32200.09.589721118-119
AAGATCG453950.09.448831114-115
CACGTCT40250.09.273576120
GAGATCG306650.08.941056120
TTAGATC106500.08.4754505116-117
CAGATCG279650.08.093927112-113
ACACGTC38050.07.847809118-119
Produced by FastQC (version 0.11.5)