FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15474069
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC205650.085.03301120
GAGCACA327350.053.285892120
AGAGCAC449700.039.118797118-119
AAGAGCA673300.026.317339118-119
GAAGAGC752200.023.368067116-117
GGAAGAG936300.019.003307116-117
CGGAAGA1094200.016.1717114-115
TCGGAAG1093100.016.131208114-115
ATCGGAA1281300.013.859087112-113
GATCGGA1323300.013.372324112-113
AGATCGG1444500.012.343822110-111
TAGATCG319700.011.62272110-111
CTAGATC70500.011.027511108-109
CACGTCT57350.010.334037120
TTAGATC106050.010.1524935118-119
GAGATCG614550.09.6278305112-113
CCTAGAT25350.09.214321118-119
CAGATCG513800.09.114549110-111
AAGATCG440300.09.060689114-115
ACGCTAG6750.009560048.671707120
Produced by FastQC (version 0.11.5)