FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14435259
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC178550.075.689316120
GAGCACA298650.046.15823120
AGAGCAC394600.034.761505118-119
AAGAGCA614200.023.292902120
GAAGAGC689250.021.640717120
GGAAGAG821550.018.650242120
CGGAAGA927150.016.404865118-119
TCGGAAG961800.015.588603118-119
ATCGGAA1130950.013.532478116-117
GATCGGA1144650.013.160536116-117
AGATCGG1271850.011.972377114-115
TAGATCG291150.011.057592114-115
CTAGATC78850.010.0465765120
AAGATCG544850.09.997144114-115
CACGTCT44100.09.825967120
ACACGTC42850.09.480568120
GAGATCG390750.09.469976112-113
TTAGATC127850.09.162271112-113
CAGATCG360600.08.918704120
CACACGT42650.08.572522120
Produced by FastQC (version 0.11.5)