FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15122902
Sequences flagged as poor quality0
Sequence length50-126
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC113150.073.840515120
GAGCACA176400.047.36425120
AGAGCAC237550.035.419025120
GAAGAGC412900.021.310642118-119
AAGAGCA432550.021.199865120
GGAAGAG547200.016.556787120
CGGAAGA634650.015.559478120
TCGGAAG631250.015.026855120
ATCGGAA783050.012.5356045118-119
GATCGGA794900.012.515085120
AGATCGG856300.011.677723120
TAGATCG229300.010.854321120
CTAGATC64400.09.519278118-119
AAGATCG415650.08.990772120
GAGATCG276350.08.474971120
TTAGATC125550.08.303833120
CAGATCG238500.08.047965114-115
CACACGT38050.07.139274120
CACGTCT47150.06.8513784120
TGAGATC162900.06.670335120
Produced by FastQC (version 0.11.5)