FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13649416
Sequences flagged as poor quality0
Sequence length54-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG64350.08.976659120
ATCTCGG499650.08.550362120
GTGTAGA745950.08.35684120
CTCGGTG443650.08.341164120
TAGATCT568200.08.323642120
CGCCGTA241550.08.245423120
GTGGTCG313750.08.227456120
AGATCTC557050.08.047374120
GGTCGCC282300.07.9930477120
GGTGGTC357850.07.9029436120
GTAGATC724800.07.7954183120
CCGTATC236700.07.7788463120
TCTCGGT460550.07.721518120
TCGGTGG417300.07.6854777120
CGGTGGT397100.07.65215120
AGTGTAG755600.07.63451118-119
GATCTCG531650.07.6169524120
TGGTCGC290000.07.594079120
GTCGCCG293350.07.4047966120
GAGTGTA809250.07.3730288118-119
Produced by FastQC (version 0.11.5)