FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15160326
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC184750.082.64667120
GAGCACA317700.047.038403120
AGAGCAC412950.036.75286118-119
AAGAGCA644950.024.025072120
GAAGAGC713700.021.53205118-119
GGAAGAG838200.018.266937120
CGGAAGA948000.016.262848116-117
TCGGAAG992250.015.6587120
ATCGGAA1156250.013.473645118-119
GATCGGA1175350.013.1677265118-119
AGATCGG1293750.012.0501585116-117
CTAGATC77950.011.687663120
TAGATCG338950.011.180324112-113
GAGATCG440100.09.963117116-117
CAGATCG520350.09.850827110-111
CCCCCCT7402.5210225E-59.1897541
TTAGATC108100.09.129743116-117
ATAGATC178500.09.060483120
AAGATCG290150.08.933006120
CACACGT49900.08.916493120
Produced by FastQC (version 0.11.5)