FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16254196
Sequences flagged as poor quality0
Sequence length54-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT283000.08.628966120
AGATCTC297100.08.219446120
GAGTGTA398650.08.202676120
TCTCGGT225050.08.184839120
AGTGTAG372850.08.046123120
ATCTCGG244500.07.8772926120
AGAGTGT442950.07.8022275120
GTGTAGA380800.07.7048244120
TCGGTGG213900.07.6577764120
TAGGGAA683700.07.6524715120
CTCGGTG216450.07.5121202120
GTAGGGA708750.07.492056120
AAGAGTG496050.07.450854120
GATCTCG277550.07.3284016120
GGGAAAG714500.07.188236120
TGTAGGG758350.07.1681867120
GTAGATC386100.07.125484118-119
GTGTAGG822850.07.0219283120
GGTGGTC182900.07.0202227120
CGTGTAG861250.06.9944777120
Produced by FastQC (version 0.11.5)