FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10373573
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC95950.062.137203120
GAGCACA144600.041.76402120
AGAGCAC191750.031.344501118-119
AAGAGCA306200.020.183758120
GAAGAGC337800.018.42863120
GGAAGAG423550.015.712852120
TCGGAAG488900.014.124503120
CGGAAGA505300.013.716883120
GATCGGA576350.012.131931118-119
ATCGGAA584550.012.120717120
AGATCGG637950.010.985427120
TAGATCG152400.010.464869116-117
CTAGATC38250.010.234799120
CACGTCT37550.010.083772120
CACACGT31850.09.1683445118-119
AAGATCG262200.08.052748120
TTAGATC60600.08.048629120
GAGATCG245200.07.826059116-117
ACACGTC39000.07.5696216120
CCTAGAT17000.07.5504613116-117
Produced by FastQC (version 0.11.5)