FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12867142
Sequences flagged as poor quality0
Sequence length50-126
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC131700.042.56373120
GAGCACA183850.031.238121120
AGAGCAC230750.024.997292120
AAGAGCA323700.018.939098120
GAAGAGC364850.016.59749120
GGAAGAG434700.015.799417120
TCGGAAG485050.014.15975118-119
CGGAAGA512850.013.574625120
GATCGGA592600.012.749633120
ATCGGAA593200.012.515502120
AGATCGG691200.010.928471118-119
TAGATCG163750.010.609538120
CTAGATC43600.09.792286116-117
TTAGATC60050.09.36618120
TCTAGAT18800.08.123036114-115
GCTAGAT19800.07.712782114-115
GCCTAGA10051.4431862E-87.461879120
AAGATCG278650.07.3336754120
CCTAGAT18700.07.333161114-115
CGCTAGA9550.00136189737.1981735120
Produced by FastQC (version 0.11.5)