FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15468557
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC169250.072.44508120
GAGCACA272300.046.950115120
AGAGCAC370000.034.244564120
AAGAGCA575100.023.80461120
GAAGAGC630000.020.969194118-119
GGAAGAG772200.018.267155120
TCGGAAG924800.015.006486118-119
CGGAAGA916850.014.997436120
GATCGGA1101800.012.966962120
ATCGGAA1103000.012.673121120
AGATCGG1221600.011.706298120
TAGATCG259950.010.63137114-115
AAGATCG553350.09.87914120
CTAGATC66700.09.704726114-115
GAGATCG392750.09.136903120
TTAGATC118900.08.856949116-117
CAGATCG375350.08.452518120
TAAGATC229350.08.335037120
CACACGT41950.07.8347573120
CACGTCT51800.07.639829120
Produced by FastQC (version 0.11.5)