FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14789545
Sequences flagged as poor quality0
Sequence length57-126
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG68750.09.77323120
TGGTCGC268200.08.723658120
TAGATCT529900.08.581595120
GTGGTCG291600.08.537945120
GGTGGTC326750.08.207005120
ATCTCGG451550.08.011323120
GATCTCG488150.07.889955120
CTCGGTG397700.07.7686296120
AGATCTC498250.07.669815120
TCGGTGG369000.07.4949255120
CGTATCA187150.07.468938120
AGAGTGT781850.07.350804120
GGTCGCC271800.07.327931120
AGTGTAG694650.07.280384120
AAGAGTG857350.07.228279120
GTAGATC666350.07.184446120
CGGTGGT364850.07.128033118-119
TCTCGGT414200.07.0680976120
GAGTGTA727050.07.038458120
GTGTAGA676400.06.9446588120
Produced by FastQC (version 0.11.5)